Cryopreservation

Cryopreservation, a complementary conservation approach to in vitro cell and tissue culture, has also been successfully employed for plant preservation at GRIPP. Cryopreservation techniques, i.e. storage at ultra-low temperature (usually in liquid nitrogen at -196°C) in principle, are applicable to any type of plant tissue with regeneration potential. This allows for the preservation of plant genetic material for future replanting efforts as well as long term storage of germplasm. Such techniques have now been developed for American elm, ginseng and many other medicinal plants in the PCTL in collaboration with GRIPP.

With the support of the Gosling Foundation, GRIPP is currently in the process of developing a brand new state-of-the-art cryopreservation facility that will house the technology and equipment needed to carry out this process and will be the new home for GRIPP’s Cryo-Bank.

Selected Publications:

Uchendu, E.E., Shukla, M.R., Reed, B.M. and Saxena, P.K. 2013. Melatonin enhances the recovery of cryopreserved shoot tips of American elm (Ulmus americana L.). J. Pineal Res. 55 (4): 435-442

Uchendu, E.E., Shukla, M.R., Reed, B.M. and Saxena, P.K. 2013. Cryopreservation of Medicinal Plants: Role of Melatonin. Acta Hort.(ISHS): In press.

Uchendu, E.E., Brown, D.C.W. and Saxena, P.K. 2011 Cryopreservation of Shoot Tips and cotyledons of the north American Genseng (Panax quinquefolius L.). CryoLetters 32 (6):463-472.